- The Pluripotency for Organ Regeneration Research Group is looking for a Laboratory Technician to develop scientific support to the research activities of the group in the framework of the project entitled "Modelling Ataxia Telangiectasia (A-T) pathogenesis and therapeutics using human pluripotent stem cells and genetic engineering". The objective of the project is to generate ATM-defective human pluripotent stem cell lines via CRISPR/Cas9 genome editing to capture the genetic background of AT patients.Main tasks and responsibilities: Maintenance of human pluripotent stem cells (culture, passage, frozen stocks preparation)Support on generation and characterization of ATM KO and KI cell lines from human pluripotent stem cellsGeneration and characterization of Cerebral Organoids from ATM KO and KI human pluripotent stem cells by RT-PCR, WB, immunofluorescence and confocal microscopy techniquesRequirements for candidates: Essential: Degree in biology, biotechnology, or similar areas.Previous experience in cell culture and human pluripotent stem cells manipulation.Previous experience in molecular biology techniques.Competencies and skills: Excellent communication and teamworking skills, proactivity, commitment, critical and analytical thinking.High level of English.Advantageous: Previous experience in CRISPR-Cas9 genome editing and in the generation of brain organoids.Previous experience in Cloning, PCR, RT-PCR, WB, Immunofluorescence and Confocal Image Processing.Previous experience in scientific support to the research activities of international or national research labs.We Offer: Number of available positions: 1Starting date: January 16th, 2025Full time 1 year labour-contract. Open-ended contract due to technical and scientific activities linked to the project and budget duration.Measures to reconcile work and family life (maternity and paternity leave, flexible schedule working hours, 23 working days of paid holidays, 9 leave days for personal matters, among others).IBEC ensures equality of access to professional development opportunities irrespective of employment status, length at IBEC or other factors.The IBEC's yearly training catalogue offers a wide range of training in technical and transferable skills including mobility grants and a Mentoring program for predoctoral and postdoctoral researchers.Stimulating, interdisciplinary research and high-quality international scientific environment.Induction program to facilitate incorporation at IBEC and additional support is provided for foreigners to obtain Visa-working permit and to install in Barcelona.How to apply: Until 10th of December an online application form is available through IBEC dedicated site:Only those applications submitted before the deadline will be evaluated.Reference: LT-EG.If you have any further question regarding your application, please contact us at ******
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